Free Tool · Works Offline

Reconstitution Calculator

Enter your peptide vial size, bacteriostatic water volume and desired dose — get your exact syringe mark instantly.

Reconstitution Calculator

Enter your peptide vial size, bacteriostatic water volume, and desired dose — get your exact syringe mark instantly.

Syringe size
Peptide vial quantity
mg
Bacteriostatic water added
ml
Dose per injection
mcg
mg
mg
Syringe capacity exceeded — use a larger syringe or split the dose.
Add to home screenWorks offline · No app store needed
Pull syringe to
IU
Complete all fields to calculate
Volume
Concentration
Doses / vial
Select values above to calculate
How to reconstitute
1
Prepare your workspace
Wipe surface with 70% isopropyl alcohol. Wash hands. Lay out peptide vial, BAC water, sterile syringes, and fresh alcohol swabs.
2
Bring to room temperature
Allow both sealed vials to warm before opening. Cold liquid on cold powder causes cloudiness and incomplete dissolution.
3
Swab both stoppers
Circular motion for 30 seconds, air dry 30 seconds. Every puncture is a contamination risk.
4
Inject slowly along the wall
Tilt vial at 45° and let water trickle down the inner glass wall. Never squirt directly onto the powder.
5
Swirl until clear
Gently swirl — never shake — until completely dissolved and solution is clear.
6
Inspect and label
Solution must be completely clear. Label every vial: date, BAC water volume, concentration.
🌀 Swirl, never shake
💉 23g recon · 27–29g inject
🔒 One syringe per use
🏷️ Label every vial
↔️ Rotate injection sites
📐 Inject at 45–90°
👁️ Check clarity before use
Storage after reconstitution
+4°C
Up to 28 days
Refrigerated, sealed, away from light
−20°C
3–4 months
Freeze in aliquots. Avoid repeat freeze-thaw.

Frequently Asked Questions

Everything you need to know about peptide reconstitution

What is peptide reconstitution?
Reconstitution is the process of dissolving a lyophilised (freeze-dried) peptide powder with bacteriostatic water (BAC water) to create an injectable solution. The amount of BAC water you add determines the concentration of your solution and therefore the volume you need to draw for each dose.
What is bacteriostatic water (BAC water)?
Bacteriostatic water is sterile water containing 0.9% benzyl alcohol, which prevents bacterial growth. It is the recommended diluent for peptide reconstitution as it keeps your solution stable for up to 28 days when refrigerated. Never use tap water or plain saline.
How much BAC water should I add to my peptide vial?
This depends on your desired dose and syringe size. Use the calculator above — enter your vial quantity, the amount of BAC water you plan to add, and your desired dose. The calculator will tell you exactly what unit mark to pull your syringe to.
What does IU mean on a syringe?
IU stands for International Units and is the measurement scale printed on insulin syringes. On a standard 1ml insulin syringe, 100 IU = 1ml. If the calculator says pull to 20 IU, that means draw to the 20 mark on your syringe — which equals 0.2ml of solution.
What needle should I use for peptide injections?
There are two steps — reconstitution and injection — and each uses a different gauge. Use a 23 gauge needle to draw up the bacteriostatic water and reconstitute your peptide (the larger gauge makes it easier to transfer liquid without damaging the rubber stopper). Then switch to a 27–29 gauge needle for the actual injection — finer, less discomfort, ideal for subcutaneous (under skin) injections at 45°. ACE Peptides ships both gauges with every order. You will also need 70% isopropyl alcohol wipes — use one to swab the rubber stopper on both your peptide vial and BAC water vial before every puncture, and a fresh one to clean the injection site before each dose. This is non-negotiable for sterile technique. Rotate injection sites between the abdomen, thigh, and upper arm to avoid tissue buildup.
How do I store reconstituted peptides?
Once reconstituted, store your peptide vial in the refrigerator at +4°C for up to 28 days. For longer storage, freeze at −20°C in individual aliquots and avoid repeated freeze-thaw cycles. Keep away from light at all times.
Can I use this calculator on my phone?
Yes — this calculator is fully mobile optimised and can be installed directly to your phone home screen as an app. On iPhone, tap Share then "Add to Home Screen". On Android, tap the browser menu and select "Add to Home Screen". It works offline once installed.
Where can I buy research peptides in the Philippines?
ACE Peptides ships research-grade lyophilised peptides across the Philippines — including Manila, Cebu, Davao, Makati, BGC, Quezon City, and all major cities — as well as Singapore, Malaysia, Thailand, and across SE Asia. Our catalogue includes popular research compounds such as Retatrutide, BPC-157, GHK-Cu, TB-500, Glow peptides, Semax, Selank, PT-141 and more, all at ≥99% purity. Visit acepeptides.health to shop the full catalogue.